Journal: Arthritis and rheumatism

Article Title: Pioglitazone, a peroxisome proliferator-activated receptor gamma agonist, reduces the progression of experimental osteoarthritis in guinea pigs.

doi: 10.1002/art.20792

Figure Lengend Snippet: Figure 4. Expression of matrix metalloproteinase 13 (MMP-13) (A–C) and interleukin-1 (IL-1) (D–F) in representative sections of cartilage from the tibial plateaus of normal (A and D), placebo-treated osteoarthritic (OA) (B and E), and pioglitazone (20 mg/kg/day)–treated OA (C and F) guinea pigs. These sections were immunostained with a polyclonal antibody against MMP-13 or IL-1. Positive cells showed dark brown staining. No specific staining was detected in cartilage treated with immunoadsorbed serum (G). Original magnification 40.

Article Snippet: Slides were further incubated with a 1.5% normal goat serum solution (Vectastain Elite ABC kit; Vector, Burlingame, CA) for 30 minutes at room temperature to suppress nonspecific binding, and were blotted and then overlaid with a rabbit polyclonal antibody against MMP-13 (1.0 mg/ml, 1:5,000; Triple Point Biologics, Portland, OR) or a rabbit polyclonal antibody against IL-1 (85 mg/ml, 1:1,700; Rockland, Gilbertsville, PA) at 4°C for 18 hours in a humidified chamber.

Techniques: Expressing, Staining